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1640培養基SH30809.01B

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品牌其他品牌貨號H4522
規格500ml供貨周期現貨
主要用途細胞培養應用領域醫療衛生,化工,生物產業,農業,石油

1640培養基SH30809.01B

品牌:Hyclone

中文名:培養基

外文名:Medium

作 用:供微生物(wu)(wu)、植(zhi)物(wu)(wu)和動物(wu)(wu)組織生長等

保(bao)存方法(fa):防潮、避光、陰涼處保(bao)存        

成 分:碳水(shui)、含氮、無機鹽、維生素、水(shui)…..

保存溫度:3~6℃

存貨:8000

1640培養基SH30809.01B配置原則

1、選擇(ze)適(shi)宜的(de)(de)(de)(de)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi):總體(ti)而言,所(suo)有(you)微(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)生(sheng)(sheng)(sheng)(sheng)(sheng)長繁殖均(jun)需(xu)要培(pei)養(yang)(yang)(yang)(yang)基含有(you)碳(tan)源(yuan)、氮源(yuan)、無(wu)(wu)機鹽、生(sheng)(sheng)(sheng)(sheng)(sheng)長因(yin)子、水及能(neng)源(yuan),但由(you)于微(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)類(lei)型復(fu)雜,不(bu)同(tong)微(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)對營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi)的(de)(de)(de)(de)需(xu)求(qiu)是(shi)不(bu)一(yi)樣的(de)(de)(de)(de),因(yin)此首先要根據(ju)不(bu)同(tong)微(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)需(xu)求(qiu)配(pei)制(zhi)針對性強的(de)(de)(de)(de)培(pei)養(yang)(yang)(yang)(yang)基。2、營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi)濃度及配(pei)比(bi)合(he)適(shi):培(pei)養(yang)(yang)(yang)(yang)基中營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi)濃度合(he)適(shi)時微(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)才能(neng)生(sheng)(sheng)(sheng)(sheng)(sheng)長良好,營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi)濃度過低時不(bu)能(neng)滿足(zu)微(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)正常(chang)生(sheng)(sheng)(sheng)(sheng)(sheng)長所(suo)需(xu),濃度過高時則(ze)可能(neng)對微(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)生(sheng)(sheng)(sheng)(sheng)(sheng)長起(qi)抑(yi)制(zhi)作用,例如(ru)高濃度糖類(lei)物(wu)(wu)(wu)(wu)質(zhi)、無(wu)(wu)機鹽、重金屬離子等不(bu)僅(jin)不(bu)能(neng)維持(chi)和促(cu)進微(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)長,反而起(qi)到抑(yi)菌(jun)(jun)或殺菌(jun)(jun)作用。3、另外,培(pei)養(yang)(yang)(yang)(yang)基中各營(ying)(ying)(ying)養(yang)(yang)(yang)(yang)物(wu)(wu)(wu)(wu)質(zhi)之(zhi)間的(de)(de)(de)(de)濃度配(pei)比(bi)也直接影(ying)響微(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)長繁殖和(或)代(dai)謝(xie)產物(wu)(wu)(wu)(wu)的(de)(de)(de)(de)形(xing)成(cheng)和積累(lei),其中碳(tan)氮比(bi)(C/N)的(de)(de)(de)(de)影(ying)響較大。4、控制(zhi)氧化還原電(dian)位(wei)(redox potential):

不同(tong)類(lei)型微(wei)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)生(sheng)(sheng)(sheng)(sheng)長對(dui)(dui)氧化還原電位(F)的(de)(de)(de)(de)(de)要(yao)求不一(yi)(yi)樣,一(yi)(yi)般好(hao)氧性(xing)微(wei)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)在(zai)(zai)(zai)F值為(wei)(wei)+0.1V以(yi)上時可(ke)正(zheng)常生(sheng)(sheng)(sheng)(sheng)長,一(yi)(yi)般以(yi)+0.3一(yi)(yi)+0.4V為(wei)(wei)宜(yi),厭氧性(xing)微(wei)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)只(zhi)能在(zai)(zai)(zai)F值低(di)于+0.1V條(tiao)件(jian)下(xia)生(sheng)(sheng)(sheng)(sheng)長,兼性(xing)厭氧微(wei)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)在(zai)(zai)(zai)F值為(wei)(wei)+0.1V以(yi)上時進(jin)行好(hao)氧呼(hu)吸,在(zai)(zai)(zai)+0.1V以(yi)下(xia)時進(jin)行發酵。F值與氧分壓和pH有關,也受某些微(wei)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)代謝產物(wu)(wu)的(de)(de)(de)(de)(de)影(ying)響。5、原料(liao)來源(yuan)的(de)(de)(de)(de)(de)選擇::在(zai)(zai)(zai)配制(zhi)培(pei)養(yang)(yang)基(ji)時應盡量(liang)利用(yong)(yong)廉價(jia)且易于獲得的(de)(de)(de)(de)(de)原料(liao)作(zuo)為(wei)(wei)培(pei)養(yang)(yang)基(ji)成(cheng)(cheng)分,特別是在(zai)(zai)(zai)發酵工業中,培(pei)養(yang)(yang)基(ji)用(yong)(yong)量(liang)很大(da),利用(yong)(yong)低(di)成(cheng)(cheng)本的(de)(de)(de)(de)(de)原料(liao)更(geng)體現出其經濟價(jia)值。6、滅菌處理(li):要(yao)獲得微(wei)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)純培(pei)養(yang)(yang),必須(xu)避免雜菌污染,因此對(dui)(dui)所(suo)用(yong)(yong)器材及工作(zuo)場所(suo)進(jin)行消毒與滅菌。對(dui)(dui)培(pei)養(yang)(yang)基(ji)而(er)(er)言(yan),更(geng)是要(yao)進(jin)行嚴格(ge)的(de)(de)(de)(de)(de)滅菌。對(dui)(dui)培(pei)養(yang)(yang)基(ji)一(yi)(yi)般采(cai)取高壓蒸汽滅菌,一(yi)(yi)般培(pei)養(yang)(yang)基(ji)用(yong)(yong)1.05kg/cm2,121.3℃條(tiao)件(jian)下(xia)維持15~30min可(ke)達到滅菌目的(de)(de)(de)(de)(de)。在(zai)(zai)(zai)配制(zhi)培(pei)養(yang)(yang)基(ji)過程中,泡沫的(de)(de)(de)(de)(de)存在(zai)(zai)(zai)對(dui)(dui)滅菌處理(li)極不利,因為(wei)(wei)泡沫中的(de)(de)(de)(de)(de)空氣形成(cheng)(cheng)隔熱層,使泡沫中微(wei)生(sheng)(sheng)(sheng)(sheng)物(wu)(wu)難以(yi)被(bei)殺死。因而(er)(er)有時需要(yao)在(zai)(zai)(zai)培(pei)養(yang)(yang)基(ji)中加入消泡沫劑以(yi)減少泡沫的(de)(de)(de)(de)(de)產生(sheng)(sheng)(sheng)(sheng),或適當提高滅菌溫度。

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