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DMEM培養基

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詳細介紹
品牌其他品牌貨號H4522
規格500ml供貨周期現貨
主要用途細胞培養應用領域醫療衛生,化工,生物產業,農業,石油

DMEM培養基

SH30022.01B        

配置原則

1、選擇適宜的(de)(de)(de)營(ying)養物(wu)(wu)(wu)(wu)質(zhi)(zhi):總體(ti)而言,所有(you)微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長繁殖(zhi)(zhi)均需(xu)要培(pei)養基含有(you)碳源、氮源、無機鹽、生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長因(yin)子、水及能(neng)源,但由于微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)營(ying)養類型復(fu)雜,不(bu)同微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)對營(ying)養物(wu)(wu)(wu)(wu)質(zhi)(zhi)的(de)(de)(de)需(xu)求是不(bu)一樣的(de)(de)(de),因(yin)此(ci)首先要根據不(bu)同微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)的(de)(de)(de)營(ying)養需(xu)求配制(zhi)針對性強的(de)(de)(de)培(pei)養基。2、營(ying)養物(wu)(wu)(wu)(wu)質(zhi)(zhi)濃度(du)及配比合(he)適:培(pei)養基中(zhong)(zhong)營(ying)養物(wu)(wu)(wu)(wu)質(zhi)(zhi)濃度(du)合(he)適時(shi)微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)才能(neng)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長良好,營(ying)養物(wu)(wu)(wu)(wu)質(zhi)(zhi)濃度(du)過(guo)低時(shi)不(bu)能(neng)滿足微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)正(zheng)常(chang)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長所需(xu),濃度(du)過(guo)高時(shi)則可能(neng)對微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長起抑制(zhi)作(zuo)用(yong)(yong),例(li)如(ru)高濃度(du)糖類物(wu)(wu)(wu)(wu)質(zhi)(zhi)、無機鹽、重(zhong)金屬離子等不(bu)僅不(bu)能(neng)維持和促進微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)的(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長,反(fan)而起到抑菌或殺菌作(zuo)用(yong)(yong)。3、另外,培(pei)養基中(zhong)(zhong)各營(ying)養物(wu)(wu)(wu)(wu)質(zhi)(zhi)之間的(de)(de)(de)濃度(du)配比也直接影響微生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)(wu)(wu)(wu)的(de)(de)(de)生(sheng)(sheng)(sheng)(sheng)(sheng)(sheng)長繁殖(zhi)(zhi)和(或)代謝產(chan)物(wu)(wu)(wu)(wu)的(de)(de)(de)形(xing)成和積累,其中(zhong)(zhong)碳氮比(C/N)的(de)(de)(de)影響較大。4、控(kong)制(zhi)氧化還原電(dian)位(redox potential):

不同類(lei)型微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)生(sheng)(sheng)(sheng)(sheng)(sheng)長對(dui)氧(yang)化還原電位(F)的(de)要(yao)求不一(yi)樣,一(yi)般好氧(yang)性微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)在(zai)(zai)F值(zhi)(zhi)為+0.1V以上時(shi)(shi)可(ke)正常生(sheng)(sheng)(sheng)(sheng)(sheng)長,一(yi)般以+0.3一(yi)+0.4V為宜,厭(yan)氧(yang)性微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)只能在(zai)(zai)F值(zhi)(zhi)低于+0.1V條件下生(sheng)(sheng)(sheng)(sheng)(sheng)長,兼性厭(yan)氧(yang)微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)在(zai)(zai)F值(zhi)(zhi)為+0.1V以上時(shi)(shi)進行(xing)(xing)(xing)好氧(yang)呼吸(xi),在(zai)(zai)+0.1V以下時(shi)(shi)進行(xing)(xing)(xing)發(fa)酵。F值(zhi)(zhi)與(yu)氧(yang)分(fen)壓(ya)和pH有關,也(ye)受某些微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)代謝產物(wu)的(de)影響。5、原料(liao)來源的(de)選擇::在(zai)(zai)配制培(pei)養(yang)(yang)基(ji)時(shi)(shi)應盡量利用(yong)廉(lian)價且易于獲(huo)得的(de)原料(liao)作為培(pei)養(yang)(yang)基(ji)成分(fen),特別是在(zai)(zai)發(fa)酵工(gong)業中,培(pei)養(yang)(yang)基(ji)用(yong)量很大,利用(yong)低成本的(de)原料(liao)更體(ti)現(xian)出其(qi)經濟價值(zhi)(zhi)。6、滅菌(jun)(jun)處理:要(yao)獲(huo)得微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)純培(pei)養(yang)(yang),必須避免雜(za)菌(jun)(jun)污染,因此對(dui)所用(yong)器材(cai)及工(gong)作場所進行(xing)(xing)(xing)消(xiao)(xiao)毒與(yu)滅菌(jun)(jun)。對(dui)培(pei)養(yang)(yang)基(ji)而(er)言,更是要(yao)進行(xing)(xing)(xing)嚴格的(de)滅菌(jun)(jun)。對(dui)培(pei)養(yang)(yang)基(ji)一(yi)般采取高壓(ya)蒸汽滅菌(jun)(jun),一(yi)般培(pei)養(yang)(yang)基(ji)用(yong)1.05kg/cm2,121.3℃條件下維(wei)持15~30min可(ke)達到滅菌(jun)(jun)目的(de)。在(zai)(zai)配制培(pei)養(yang)(yang)基(ji)過程(cheng)中,泡沫(mo)的(de)存在(zai)(zai)對(dui)滅菌(jun)(jun)處理極不利,因為泡沫(mo)中的(de)空氣形(xing)成隔(ge)熱層,使泡沫(mo)中微(wei)(wei)(wei)生(sheng)(sheng)(sheng)(sheng)(sheng)物(wu)難以被殺死(si)。因而(er)有時(shi)(shi)需要(yao)在(zai)(zai)培(pei)養(yang)(yang)基(ji)中加入消(xiao)(xiao)泡沫(mo)劑以減少(shao)泡沫(mo)的(de)產生(sheng)(sheng)(sheng)(sheng)(sheng),或適當提高滅菌(jun)(jun)溫度。

DMEM培養基

SH30022.01B企業同類產品:

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SH30024.01B   MEM液體培養基   MEM       ; HyClone   500ml

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